rat tyrosine hydroxylase (TH) promoter, rabbit beta-globin intron, jelly fish green fluorescent protein (GFP) gene, rabbit beta-globin poly A, SV40 early gene poly A ラットチロシン水酸化酵素 (TH) 遺伝子プロモーターに、オワンクラゲ緑色蛍光タンパク質 (GFP) 遺伝子を接続した導入遺伝子を持つ。Tgマウスは脳内および末梢器官のカテコールアミン合成細胞がGFPにより標識される。ホモマウスは致死 (原因は不明) 。 条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。J. Neurochem., 82, 295-304 (2002). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurochem., 82, 295-304 (2002). <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/may_2008_mm" target="_blank">Mouse of the Month May 2008</A><br><a href="https://mus.brc.riken.jp/ja/wp-content/uploads/pdf/blc/02095_GB.pdf">Genetic Background</a> B6.Cg-Tg(TH-GFP)21-31 Carrier x Noncarrier [C57BL/6JJcl] Carrier x Noncarrier [C57BL/6JJcl] 小林　和人 Fluorescent Proteins/lacZ System Developed by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J. Two lines were generated. RBRC02095 (commonly-used), RBRC03162. Kazuto KOBAYASHI RBRC02095 B (1-3 months) <a href='https://brc.riken.jp/mus/pcr02095'>Genotyping protocol -PCR-</a> 公立大学法人福島県立医科大学 The TH-GFP transgenic mouse was generated to express GFP in the majority of midbrain dopamine neurons under the control of the rat TH gene promoter. Dopamine is a neurotransmitter which mediates motor control, cognition, emotion, reward, and neuroendocrine functions. Dysfunction of dopamine-producing neurons in the midbrain is involved in some neurological and neuropsychiatric diseases such as Parkinson’s disease and schizophrenia. These dopamine neurons express tyrosine hydroxylase (TH), a key enzyme of catecholamine biosynthesis. This transgenic mouse is useful for visualizing dopamine neurons to study the physiology and pathogenesis of dopamine neurons. Homozygous transgenic mice are lethal. Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol><A HREF="https://www.fmu.ac.jp/cms/molgenet/index_html" target="_blank">Lab HP (Japanese)</A> B（1〜3か月） <a href="https://www.brc.riken.jp/lab/animal/images/02095_map.jpg">Construct map</a><br><a href="https://www.brc.riken.jp/lab/animal/info/02095_seq.doc">Transgene sequence</a> 福島県立医科大学医学部・小林和人先生（1998）。B6D2F2受精卵へインジェクションにより作出。C57BL/6Jに戻し交配された。2ラインあり。RBRC02095（メインライン）、RBRC03162。 Fukushima Medical Univ. true B6.B6D2-Tg(Th-EGFP)21-31Koba B6.B6D2-Tg(Th-EGFP)21-31Koba Neurobiology Research